| 张振冬,王淑芬,巩宁,王秀娟,朱琳.牙鲆红细胞对不同抗原的免疫黏附及Ⅰ型补体受体的检测[J].高技术通讯(中文),2010,20(3):320~326 |
| 牙鲆红细胞对不同抗原的免疫黏附及Ⅰ型补体受体的检测 |
| Paralichthys olivaceus erythrocytes’ immune adherence to different antigens and detection of their complement receptor Ⅰ |
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| DOI： |
| 中文关键词: 牙鲆， 免疫黏附， Ⅰ型补体受体(CR1)， C3b受体花环， 红细胞免疫 |
| 英文关键词: Paralichthys olivaceus, immune adherence, complement receptor I (CR1), C3b receptor rosette, erythrocyte immune |
| 基金项目:国家自然科学基金(40606030)和863计划(2006AA09Z170)资助项目 |
| 作者 | 单位 | | 张振冬 | 国家海洋环境监测中心大连 | | 王淑芬 | 大连水产学院生命科学与技术学院 | | 巩宁 | 大连海事大学环境系统生物学研究所 | | 王秀娟 | 南开大学环境科学与工程学院天津 | | 朱琳 | 南开大学环境科学与工程学院天津 |
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| 中文摘要: |
| 采用C3b受体花环实验探究了牙鲆红细胞对不同种类抗原的免疫黏附能力，确定了影响免疫黏附作用的理化因子。实验选取创伤弧菌、金黄色葡萄球菌和啤酒酵母3种不同类别的抗原分别与牙鲆红细胞在优化条件下反应。结果表明，牙鲆红细胞对3种抗原均有免疫黏附作用，在20℃、01mol/L PBS++缓冲液的反应条件下，牙鲆红细胞黏附创伤弧菌、金黄色葡萄球菌和啤酒酵母的C3b受体花环率分别为(1900±101)%、(609±136)%和(342±000)%，对3种抗原的免疫黏附活性差异显著(P<005)。利用A |
| 英文摘要: |
| The C3b receptor rosette technique was employed to investigate the erythrocytes immune adherence activity of left eyed flounder (Paralichthys olivaceus) to different antigens under the optimized immune adherence conditions. Three different kinds of antigens (gram negative bacteria, gram positive bacteria and eukaryote) were chosen to incubate with erythrocytes in the 0.1 mol/L PBS++ buffer at 20℃ for 30 min. The results indicated that the flounder erythrocytes were able to adhere to all the three kinds of antigens with the C3b receptor rosette rates for Vibrio vulnificus, Staphylococcus aureus and Saccharomyces cerevisiae being (19.00±1.01)%, (6.09±1.36)% and (3.42 ± 0.00)% respectively. The external structures of the antigens were inferred to be responsible for the significant difference of rosette rates. Furthermore, erythrocyte membrane proteins were extracted and purified by the Superdex 200 FPLC gel chromatography to prepare the complement receptorⅠ (CR1) component. The Dot ELISA assay confirmed there was the CR1 component in erythrocyte membrane proteins and the CR1 molecule homologous to higher vertebrate was expressed on erythrocytes of left eyed flounder. Erythrocytes have been proved to possess key immune molecules and play an important role in the left eyed flounder immune system in the study. |
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