张于光1刘学端2李迪强1.基因指纹和芯片技术在生物资源调查和标本整理中的应用[J].中国科技资源导刊,2010,(2):69~73 |
基因指纹和芯片技术在生物资源调查和标本整理中的应用 |
Application of Gene Fingerprint and Biochip Techniques in Biological Resource Investigation and Specimen Inventory |
投稿时间:2009-02-23 |
DOI: |
中文关键词: 基因指纹;基因芯片;生物资源调查;标本整理 |
英文关键词: gene fingerprint, biochip, biology resource investigation, specimen inventory |
基金项目:国家科技基础条件平台建设项目(2005DKA21404)。 |
作者 | 单位 | 张于光1刘学端2李迪强1 | 1. 中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京100091 2. 中南大学资源加工与生物工程学院,湖南长沙 410083 |
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中文摘要: |
随着PCR 技术的广泛应用,基因指纹和芯片技术得到快速发展,已成为分子生物学研究的重要手段并得到广泛应用。文章首先综述了目前常用的基因指纹和芯片技术及其优缺点。常用的技术有:DNA 限制性片断长度多态性分析(RFLP)、随机扩增多态性DNA 分析(RAPD)、扩增片断长度多态性分析(AFLP)、微卫星DNA 多态性分析(SSR)、单核苷酸多态性分析(SNP)等。然后简要概述这些技术在生物资源调查和标本整理中的应用。主要应用于物种鉴定和分类、个体识别和种群数量调查、资源植物关键信息研究等。最后展望这些技术在 |
英文摘要: |
With the development of PCR technique, gene fingerprint and biochip techniques have a rapid evolution and become one of the important tools in molecular biology study. At present, the gene fingerprint techniques including: DNA restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR) and single nucleotide polymorphism (SNP), and so on. The microarray is a powerful genomic tool for the characteristics of high density, high sensitivity and automation. In this paper, the authors reviewed the basic theory and characteristics of the general gene fingerprint and biochip techniques and their application in biology resource investigation and specimen inventory. At present, the major studies in biology resource investigation and specimen inventory include: species identification and classification, individual identification and population number investigation, and the key information research on resource plant. |
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