| Jiang Yong(姜勇),Zhang Xuecheng,Sun Pingnan,Chen Yunsong.[J].高技术通讯(英文),2012,18(1):107~111 |
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| Effects of oral administration recombinant Saccharomyces cerevisiae expressing human-salmon calcitonin on hypercalcemia rats① |
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| DOI： |
| 中文关键词: |
| 英文关键词: recombinant human-salmon calcitonin, immunol fluorescence, FACS, osteoclast, hypercalcemia, saccharomyces cerevisiae |
| 基金项目: |
| Author Name | Affiliation | | Jiang Yong(姜勇) | | | Zhang Xuecheng | | | Sun Pingnan | | | Chen Yunsong | |
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| 中文摘要: |
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| 英文摘要: |
| Recombinant human-salmon calcitonin (hsCT) was synthesized and expressed on the cell surfaces of recombinant Saccharomyces cerevisiae （yAGA2-hsCT）using the pAGA2-hsCT expression vector. Expression of recombinant hsCT was verified by flow cytometer.The resorption activity of osteoclasts was inhibited by recombinant hsCT protein which released from the cell surface of yAGA2-hsCT. Blood calcium determination in rats showed the activity of yAGA2-hsCT following oral administration of 5 mg/kg lyophilized transformant yeast to hypercalcemia rats decreases the serum calcium from 2.82 ± 0.023 mM to 2.662 ± 0.012 mM. |
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